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Tn7_ENDPaCFI
(Plasmid #260818)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 260818 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    pUCminiTn7
  • Vector type
    Bacterial Expression
  • Selectable markers
    Gentamicin

Growth in Bacteria

  • Bacterial Resistance(s)
    Gentamicin, 10 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    Grow at slightly lower gentamicin concentrations, at around 10µg/ml. Longer growth times are needed.
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    END(PaCFI)
  • Species
    Pseudomonas aeruginosa

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Tn7_ENDPaCFI was a gift from Joseph Bondy-Denomy (Addgene plasmid # 260818 ; http://n2t.net/addgene:260818 ; RRID:Addgene_260818)

  • For your References section:

    END nucleases are antiphage defence systems targeting multiple phages with modified genomes. Yee WX, Lee YJ, Makarova KS, Klein TA, Wirganowicz A, Gabagat AE, Liu M, Csorgo B, Koonin EV, Weigele PR, Bondy-Denomy J. Nat Microbiol. 2026 Jun 26. doi: 10.1038/s41564-026-02407-2. 10.1038/s41564-026-02407-2 PubMed 42362811
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