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pPF3929
(Plasmid #254228)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 254228 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    pC020
  • Backbone manufacturer
    Addgene Plasmid #91910
  • Backbone size (bp) 7681
  • Modifications to backbone
    Insertion of RP4 oriT
  • Vector type
    CRISPR ; LseCas13a expression backbone for spacer cloning
  • Promoter Lac promoter for LseCas13a and JS23119 promoter for CRISPR array

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol, 25 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Cloning Information

  • Cloning method Gibson Cloning

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.64898/2025.12.19.695335 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pPF3929 was a gift from Peter Fineran (Addgene plasmid # 254228 ; http://n2t.net/addgene:254228 ; RRID:Addgene_254228)
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