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pPF4213
(Plasmid #254224)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 254224 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    pBAD30 - pBAD promoter components, pSEVA121 - RK2 oriV and oriT components, pKRCPN2 - tn5 transposase and transposon components
  • Total vector size (bp) 7747
  • Modifications to backbone
    The backbone consist of a tn5 transposon and the tn5 transposase tnpA regulated by a pBAD promoter, a tetracycline resistance marker and an RK2 oriV and oriT.
  • Vector type
    Transposon insertion mutagenesis vector

Growth in Bacteria

  • Bacterial Resistance(s)
    Tetracycline, 10 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    AcrVIA1
  • Species
    Listeria seeligeri LS46 prophage
  • Insert Size (bp)
    699
  • Mutation
    Codon optimized for E. coli.
  • Promoter js23100

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.64898/2025.12.19.695335 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pPF4213 was a gift from Peter Fineran (Addgene plasmid # 254224 ; http://n2t.net/addgene:254224 ; RRID:Addgene_254224)
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