Defining the essential genome of diverse phages with phage Tn-seq.
Kyte N, Fuchs M, Smith LM, Fineran PC
Nat Microbiol. 2026 Sep 25. doi: 10.1038/s41564-026-02486-1.
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Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 254224 | pPF4213 | Mutagenesis plasmid encoding tnpA and the tn5 transposon harbouring AcrVIA1 |
| 254225 | pPF4354 | Mutagenesis plasmid encoding tnpA fused to PCH45 uvsX gene and the tn5 transposon harbouring AcrVIA1 |
| 254226 | pPF4481 | Mutagenesis plasmid encoding tnpA and the tn5 transposon harbouring AIcrVIA3 |
| 254228 | pPF3929 | LseCas13a expression plasmid including a CRISPR array for insertions of new spacers via BsmBI |