pRep2-LICA1
(Plasmid
#254237)
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PurposeRep2-LICA1 capsid plasmid (9rh74_4um9)
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Depositing Lab
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Depositing OrganizationGenethon
Located in France -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 254237 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
Backbone
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Vector backbonepUC/pBluescript
- Total vector size (bp) 7357
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Vector typeMammalian Expression, AAV, Synthetic Biology
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameTGFB3
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SpeciesH. sapiens (human)
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MutationTDGRGDLGRLGP peptide replacing the VR4 of hybrid capsid Cap9-rh74
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GenBank IDNM_003239.5
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Entrez GeneTGFB3 (a.k.a. ARVD, ARVD1, LDS5, RNHF, TGF-beta3)
Cloning Information
- Cloning method Golden Gate
- 5′ sequencing primer CGTGGGTCGTTCGTCC
- (Common Sequencing Primers)
Resource Information
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pRep2-LICA1 was a gift from Isabelle Richard (Addgene plasmid # 254237 ; http://n2t.net/addgene:254237 ; RRID:Addgene_254237) -
For your References section:
An engineered AAV targeting integrin alpha V beta 6 presents improved myotropism across species. Vu Hong A, Suel L, Petat E, Dubois A, Le Brun PR, Guerchet N, Veron P, Poupiot J, Richard I. Nat Commun. 2024 Sep 11;15(1):7965. doi: 10.1038/s41467-024-52002-4. 10.1038/s41467-024-52002-4 PubMed 39261465