Skip to main content
Addgene
Open Menu

HisGB1-CITED2(216-270) + CBP TAZ1(340-439)
(Plasmid #254287)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 254287 Standard format: Plasmid sent in bacteria as agar stab 1 $89
Add to Cart

Backbone

  • Vector backbone
    pET22b
  • Backbone manufacturer
    Novagen
  • Backbone size w/o insert (bp) 5500
  • Total vector size (bp) 6157
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert 1

  • Gene/Insert name
    CITED2 (216-270)
  • Alt name
    P35SRJ
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    165
  • Entrez Gene
    CITED2 (a.k.a. ASD8, MRG-1, MRG1, P35SRJ, VSD2)
  • Promoter T7
  • Tag / Fusion Protein
    • 6X His-tagged GB1 (N terminal on insert)

Cloning Information for Gene/Insert 1

Gene/Insert 2

  • Gene/Insert name
    CBP TAZ1 (340-439)
  • Alt name
    CBP CH1
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    303
  • Entrez Gene
    Crebbp (a.k.a. CBP, CBP/p300, KAT3A, p300/CBP)
  • Promoter T7

Cloning Information for Gene/Insert 2

  • Cloning method Restriction Enzyme
  • 5′ cloning site NcoI (not destroyed)
  • 3′ cloning site HindIII (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer T7
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Modified from Addgene Plasmid #173761 from Berlow, et al. Nature 2017. doi: 10.1038/nature21705.

Please visit https://doi.org/10.64898/2026.01.20.700670 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    HisGB1-CITED2(216-270) + CBP TAZ1(340-439) was a gift from Rebecca Berlow (Addgene plasmid # 254287 ; http://n2t.net/addgene:254287 ; RRID:Addgene_254287)

  • For your References section:

    Sequence-encoded differences in the conformational ensembles of CITED transcriptional activation domains impact coactivator binding. Do TU, Kraft EJ, Chappell GF, Parnham S, Berlow RB. bioRxiv [Preprint]. 2026 Jan 21:2026.01.20.700670. doi: 10.64898/2026.01.20.700670. 10.64898/2026.01.20.700670 PubMed 41648556
Related items: