Avi-TAZ1
(Plasmid
#254289)
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PurposeBacterial expression of His- and Avi-tagged CBP TAZ1
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 254289 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 | |
Backbone
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Vector backbonepET-21a(+)
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Backbone manufacturerEMD Biosciences
- Backbone size w/o insert (bp) 5443
- Total vector size (bp) 5803
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameCBP TAZ1 (340-439)
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Alt nameCBP CH1
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SpeciesM. musculus (mouse)
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Insert Size (bp)303
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Entrez GeneCrebbp (a.k.a. CBP, CBP/p300, KAT3A, p300/CBP)
- Promoter T7
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Tag
/ Fusion Protein
- 8X His-tag + Avi-tag (N terminal on insert)
Cloning Information
- Cloning method Gene Synthesis
- 5′ sequencing primer T7
- 3′ sequencing primer T7
- (Common Sequencing Primers)
Resource Information
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://doi.org/10.64898/2026.01.20.700670 for bioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Avi-TAZ1 was a gift from Rebecca Berlow (Addgene plasmid # 254289 ; http://n2t.net/addgene:254289 ; RRID:Addgene_254289) -
For your References section:
Sequence-encoded differences in the conformational ensembles of CITED transcriptional activation domains impact coactivator binding. Do TU, Kraft EJ, Chappell GF, Parnham S, Berlow RB. bioRxiv [Preprint]. 2026 Jan 21:2026.01.20.700670. doi: 10.64898/2026.01.20.700670. 10.64898/2026.01.20.700670 PubMed 41648556