|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||25438||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 6400
Vector typeMammalian Expression, Lentiviral
Growth in Bacteria
Insert Size (bp)330
- Cloning method Restriction Enzyme
- 5′ cloning site XhoI (destroyed during cloning)
- 3′ cloning site EcoRI (not destroyed)
- 5′ sequencing primer n/a
- 3′ sequencing primer n/a (Common Sequencing Primers)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pMA2780 was a gift from Mikhail Alexeyev (Addgene plasmid # 25438 ; http://n2t.net/addgene:25438 ; RRID:Addgene_25438)
For your References section:A retro-lentiviral system for doxycycline-inducible gene expression and gene knockdown in cells with limited proliferative capacity. Alexeyev MF, Fayzulin R, Shokolenko IN, Pastukh V. Mol Biol Rep. 2010 Apr . 37(4):1987-91. 10.1007/s11033-009-9647-7 PubMed 19655272