pCambia2300-Aar1-ccdB-GmCH
(Plasmid
#254405)
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Purpose(Empty Backbone) Gateway-compatible destination vector for bifluorescence complementation (BiFC). The plasmid carries Gus-mCherry expressed from the Arabidopsis Ubiquitin 10 promoter.
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 254405 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 | |
Backbone
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Vector backbonepCambia2300
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Modifications to backboneAddition of Ubiquitin promoter -GUSmCherry fusion and cloning sites
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Vector typePlant Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)ccdB Survival
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Copy numberHigh Copy
Cloning Information
- Cloning method Gateway Cloning
Resource Information
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCambia2300-Aar1-ccdB-GmCH was a gift from Maria Harrison (Addgene plasmid # 254405 ; http://n2t.net/addgene:254405 ; RRID:Addgene_254405) -
For your References section:
Receptor-associated kinases control the lipid provisioning program in plant-fungal symbiosis. Ivanov S, Harrison MJ. Science. 2024 Jan 26;383(6681):443-448. doi: 10.1126/science.ade1124. Epub 2024 Jan 25. 10.1126/science.ade1124 PubMed 38271524