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Addgene

pEAK10-His-Myc-Kal7
(Plasmid #25454)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 25454 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pEAK-His-Myc
  • Backbone size w/o insert (bp) 6000
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Kal7
  • Alt name
    Kalirin 7
  • Species
    R. norvegicus (rat)
  • Insert Size (bp)
    5000
  • Tags / Fusion Proteins
    • His (N terminal on insert)
    • Myc (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NcoI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer EF-1a Forward
  • 3′ sequencing primer N/A
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

There are 2 mutations in the sequence, T64S and N80Y. These sequencing errors are the result of mistakes in initial submission to GenBank. The sequence should be correct.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEAK10-His-Myc-Kal7 was a gift from Betty Eipper (Addgene plasmid # 25454 ; http://n2t.net/addgene:25454 ; RRID:Addgene_25454)
  • For your References section:

    An isoform of kalirin, a brain-specific GDP/GTP exchange factor, is enriched in the postsynaptic density fraction. Penzes P, Johnson RC, Alam MR, Kambampati V, Mains RE, Eipper BA. J Biol Chem. 2000 Mar 3. 275(9):6395-403. 10.1074/jbc.275.9.6395 PubMed 10692441