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(pRZ294) AAV: U6-gRNA-EF1a-ZsGreen
(Plasmid #254586)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 254586 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    AAV
  • Backbone size w/o insert (bp) 3700
  • Vector type
    AAV

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    sgRNA with SapI spacer
  • Alt name
    single guide RNA
  • gRNA/shRNA sequence
    GGAAGAGCGAGCTCTTCTGTTTAAGAGCTATGCTGGAAACAGCATAGCAAGTTTAAATAAGGCTAGTCCGTTATCAACTTGAAAAAGTGGCACCGAGTCGGTGC
  • Species
    Unspecified
  • Insert Size (bp)
    104

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    (pRZ294) AAV: U6-gRNA-EF1a-ZsGreen was a gift from Jesse Engreitz (Addgene plasmid # 254586 ; http://n2t.net/addgene:254586 ; RRID:Addgene_254586)
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