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pVN001
(Plasmid #254593)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 254593 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pOA-RQ
  • Backbone manufacturer
    GeneArt (Thermo Fisher Scientific)
  • Backbone size w/o insert (bp) 2414
  • Total vector size (bp) 3391
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Bacterial transcription unit encoding P-gre promoter and Broccoli-tRNA fluorogenic aptamer
  • Species
    Synthetic
  • Insert Size (bp)
    976
  • Promoter P-gre

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SfiI (not destroyed)
  • 3′ cloning site SfiI (not destroyed)
  • 5′ sequencing primer TGTAAAACGACGGCCAGT
  • 3′ sequencing primer GAAACAGCTATGACCATGTTAATGCA
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.64898/2026.02.09.704077 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pVN001 was a gift from Georgi Belogurov (Addgene plasmid # 254593 ; http://n2t.net/addgene:254593 ; RRID:Addgene_254593)

  • For your References section:

    Regulatory landscapes and structural choreography of transcription initiation in spirochetes. Trapp VK, Wang T, Hilal T, Makinen JJ, Kotikoski J, Tavi PJ, Levola V, Paavilainen S, Loll B, Wahl MC, Belogurov GA. bioRxiv 2026.02.09.704077; doi: 10.64898/2026.02.09.704077 10.64898/2026.02.09.704077
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