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Regulatory landscapes and structural choreography of transcription initiation in spirochetes

Trapp VK, Wang T, Hilal T, Makinen JJ, Kotikoski J, Tavi PJ, Levola V, Paavilainen S, Loll B, Wahl MC, Belogurov GA
bioRxiv 2026.02.09.704077; doi: 10.64898/2026.02.09.704077 (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
254593pVN001Encodes a 0.8 kb bacterial transcription unit with Spirochaeta africana P-gre promoter and Broccoli-tRNA fluorogenic aptamer shortly upstream of the intrinsic terminator
254594pVN003Encodes a 0.8 kb bacterial transcription unit with Spirochaeta africana P-rrna promoter and Broccoli-tRNA fluorogenic aptamer shortly upstream of the intrinsic terminator
254595pJK003Encodes a 0.8 kb bacterial transcription unit with Spirochaeta africana P-prs promoter and Broccoli-tRNA fluorogenic aptamer shortly upstream of the intrinsic terminator
254596pJK006Encodes a 0.8 kb bacterial transcription unit with Spirochaeta africana P-trna promoter and Broccoli-tRNA fluorogenic aptamer shortly upstream of the intrinsic terminator
254597pVL017Encodes a 0.8 kb bacterial transcription unit with Spirochaeta africana P-fla promoter and Broccoli-tRNA fluorogenic aptamer shortly upstream of the intrinsic terminator
254598pVL019Encodes a 0.8 kb bacterial transcription unit with Spirochaeta africana P-pori promoter and Broccoli-tRNA fluorogenic aptamer shortly upstream of the intrinsic terminator
254599pJK004Encodes a 0.8 kb bacterial transcription unit with Escherichia coli rrnB P1 promoter and Broccoli-tRNA fluorogenic aptamer shortly upstream of the intrinsic terminator

Antibodies from Article