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SP-cTAT-zsGreen-IRES-H2B-tdTomato
(Plasmid #254722)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 254722 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pXPR
  • Backbone size w/o insert (bp) 5481
  • Total vector size (bp) 9882
  • Vector type
    Mammalian Expression, Bacterial Expression
  • Selectable markers
    tdTomato fluorescent expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    SP-cTAT-zsGreen-IRES-H2B-tdTomato
  • Species
    Synthetic
  • Insert Size (bp)
    4401
  • Promoter PGK

Cloning Information

  • Cloning method Golden Gate
  • 5′ sequencing primer ATGCACaGGATCCagcaagagctatctacgggctagcatcGGAG
  • 3′ sequencing primer ATGCACaGGATCCagcaagagctatctacgggctagcatcGGAG
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    SP-cTAT-zsGreen-IRES-H2B-tdTomato was a gift from Judith Agudo (Addgene plasmid # 254722 ; http://n2t.net/addgene:254722 ; RRID:Addgene_254722)

  • For your References section:

    A glucocorticoid-FAS axis controls immune evasion during metastatic seeding. Cassandras M, Sanchez X, Hsu L, Huang Y, Getzler AJ, Ganguly D, Baldominos P, Codinachs I, Chuong J, Martin EE, Smith BE, Marina E, Spasic M, Qin X, Parsons HA, Mayer EL, Sarosiek KA, Dougan SK, Mittendorf EA, McAllister SS, Hsu YC, Agudo J. Nature. 2026 Mar 4. doi: 10.1038/s41586-026-10222-2. 10.1038/s41586-026-10222-2 PubMed 41781620
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