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pAAV-neuro-seeERv1.0
(Plasmid #254751)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 254751 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    pAAV
  • Backbone size w/o insert (bp) 2868
  • Total vector size (bp) 7212
  • Vector type
    Mammalian Expression, AAV

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Growth instructions
    Due to repetitive sequence, recombination is common between polyA sequences. Propagation as bacterial glycerol stocks is recommended with period checks to ensure validity before viral production. pAAV-neuro-seeERv1.1 was designed to fix this issue. This plasmid is provided as the original used to produce AAV for the publication.
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    Estrogen Response Element
  • Alt name
    ERE
  • Species
    Synthetic
  • Insert Size (bp)
    190
  • Tag / Fusion Protein
    • d2EGFP (C terminal on insert)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.64898/2025.12.14.694224 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAAV-neuro-seeERv1.0 was a gift from J Edward van Veen (Addgene plasmid # 254751 ; http://n2t.net/addgene:254751 ; RRID:Addgene_254751)

  • For your References section:

    A bifunctional optical reporter for tracking estrogen response dynamics in neurons. Cara AL, Guthman EM, Mishra A, Hall AL, Yokoyama S, Sandoval NP, Gregg I, Aghi K, Soma KK, Correa SM, Falkner AL, van Veen JE. bioRxiv 2025.12.14.694224 10.64898/2025.12.14.694224
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