Skip to main content
Addgene
Open Menu

Tol2-LyzC-CoCHR-mcherry
(Plasmid #254785)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 254785 Standard format: Plasmid sent in bacteria as agar stab 1 $94
Add to Cart

Backbone

  • Vector backbone
    Tol2-lyzc
  • Vector type
    Zebrafish Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    CoCHR-mCherry
  • Species
    Synthetic
  • Promoter Lyzc

Cloning Information

  • Cloning method Other

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Isaac Bianco, CoCHR from Addgene #124233

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Infusion Cloning. Please visit https://doi.org/10.1101/2025.03.06.641746 for bioRxiv preprint.

Please note that Addgene's sequencing results differ from the full sequence information provided by the depositing laboratory, finding H30R in mCherry compared to the reference sequence. The depositor confirms that this change is not known to affect plasmid function.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Tol2-LyzC-CoCHR-mcherry was a gift from Qing Deng (Addgene plasmid # 254785 ; http://n2t.net/addgene:254785 ; RRID:Addgene_254785)

  • For your References section:

    Inwardly rectifying potassium channels promote directional sensing during neutrophil chemotaxis. Wang T, Kim DH, Ding C, Wang D, Zhang W, Silic M, Cheng X, Shao K, Ku T, Zheng C, Xie J, Xiao S, Jayant K, Yuan C, Chubykin AA, Staiger CJ, Zhang G, Deng Q. J Cell Biol. 2026 Jan 5;225(1):e202503037. doi: 10.1083/jcb.202503037. Epub 2025 Nov 19. 10.1083/jcb.202503037 PubMed 41259080
Related items: