Skip to main content
Addgene
Open Menu

pMT-ef1a-h2b-mCherry
(Plasmid #254876)

Print
  • Purpose
    This plasmid is designed for Tol2-mediated transgenesis in zebrafish and drives ubiquitous nuclear tagged expression of mCherry2, under the control of an ef1a (eef1a1l1) derived promoter.
  • Depositing Lab
    Dan Wagner
  • Depositing Organization
    University of California, San Francisco (UCSF)
    Located in United States of America
  • Publication
    eef1a1l1:h2b-mCherry2 (unpublished) ( How to cite )
  • Sequence Information

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 254876 Standard format: Plasmid sent in bacteria as agar stab 1 $94
Add to Cart

Backbone

  • Vector backbone
    pMT- eef1a1l1:sfGFP
  • Backbone manufacturer
    Dan Wagner
  • Backbone size w/o insert (bp) 5800
  • Total vector size (bp) 6889

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    h2b-mCherry2
  • Alt name
    mCherry
  • Species
    Synthetic
  • Insert Size (bp)
    1100
  • Promoter eef1a1l1

Cloning Information

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Nicole Suren, Wagner Lab

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMT-ef1a-h2b-mCherry was a gift from Dan Wagner (Addgene plasmid # 254876 ; http://n2t.net/addgene:254876 ; RRID:Addgene_254876)
Related items: