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CHAC1-promoter-MUT-GFP
(Plasmid #255524)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 255524 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    pEGFP-N1
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 3598
  • Modifications to backbone
    In the vector pEGFP-N1, partial f1 single-strand DNA origin was removed by SspI digestion followed by self-ligation. In addition, the CMV promoter was removed by AseI and NheI digestion, followed by Klenow fragment filling and self-ligation. The modified pEGFP-N1 vector contains no f1 and CMV promoter.
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    CHAC1 promoter
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    589
  • Mutation
    ATF/CRE motif mutated
  • Tag / Fusion Protein
    • EGFP (C terminal on backbone)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Sequence inserted at EcoRI (5') and BamHI (3') sites, both preserved after cloning.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    CHAC1-promoter-MUT-GFP was a gift from Xingguo Zhu (Addgene plasmid # 255524 ; http://n2t.net/addgene:255524 ; RRID:Addgene_255524)
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