pAAV-GfaABC1D::yellow-pre-eGRASP
(Plasmid
#255751)
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PurposeGfaABC1D promoter driven expression of yellow-pre-eGRASP(p32); allows labeling of astrocytic contact with post synapses.
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Depositing Lab
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Depositing OrganizationCenter for Neurogenomics and Cognitive Research
Located in the Netherlands -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 255751 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
Backbone
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Vector backbonepAAV
- Backbone size w/o insert (bp) 4284
- Total vector size (bp) 5634
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Vector typeMammalian Expression, AAV
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameYellow pre-eGRASP(p32)
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Insert Size (bp)1350
- Promoter GfaABC1D
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer -
- (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made bypAAV-CaMKII::yellow-pre-eGRASP (gift from Bong-Kiun Kaang, Addgene plasmid #111580) & pAAV-GFAP-EGFP (gift from Bryan Roth, Addgene plasmid #50473).
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pAAV-GfaABC1D::yellow-pre-eGRASP was a gift from Mark Verheijen (Addgene plasmid # 255751 ; http://n2t.net/addgene:255751 ; RRID:Addgene_255751) -
For your References section:
Reduced astroglial-synapse coverage on cortical non-engram cells shapes remote memory strength. Mak A, Boers-Escuder C, Parisi S, Blok TM, Smit AB, van den Oever MC, Verheijen MHG. Cell Rep. 2026 Sep 1;45(9):117828. doi: 10.1016/j.celrep.2026.117828. 10.1016/j.celrep.2026.117828 PubMed 42678807