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pAAV-GfaABC1D-igκ-αGFP-TM-mScarlet
(Plasmid #259746)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 259746 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    pAAV
  • Backbone size w/o insert (bp) 4755
  • Total vector size (bp) 6135
  • Vector type
    AAV

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    igκ-αGFP-TM-mScarlet
  • Alt name
    igk
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1380
  • Entrez Gene
    IGK (a.k.a. IGK@)
  • Promoter GfaABC1D
  • Tags / Fusion Proteins
    • HA (C terminal on insert)
    • vhhGFP (C terminal on insert)
    • PDGFR TM (C terminal on insert)
    • mScarlet (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Nhe1 (not destroyed)
  • 3′ cloning site BsrG1 (not destroyed)
  • 5′ sequencing primer GfaABC1D-F
  • 3′ sequencing primer WPRE-R
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAAV-GfaABC1D-igκ-αGFP-TM-mScarlet was a gift from Sangkyu Lee (Addgene plasmid # 259746 ; http://n2t.net/addgene:259746 ; RRID:Addgene_259746)

  • For your References section:

    Remodeling synaptic connections via engineered neuron-astrocyte interactions. Kim SH, Won W, Kim GH, Kook YH, Son S, Choi S, Kang DY, Park MG, Choi YJ, Won SS, Shin J, Jeong Y, Lee KJ, Lee CJ, Lee S. Nat Commun. 2026 Apr 15;17(1):3490. doi: 10.1038/s41467-026-71440-w. 10.1038/s41467-026-71440-w PubMed 41986345
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