pAAV-CaMKIIα-igκ-mEGFP-TM
(Plasmid
#259745)
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PurposeAn AAV expression vector designed to target the SynTrogo ligand specifically to excitatory neurons in vivo under the control of the CaMKIIα promoter.
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Depositing Lab
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Depositing OrganizationInstitute for Basic Science
Located in Republic of Korea -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 259745 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
Backbone
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Vector backbonepAAV
- Backbone size w/o insert (bp) 4439
- Total vector size (bp) 5432
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Vector typeAAV
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature30°C
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Growth Strain(s)NEB Stable
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameigκ-mEGFP-TM
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Alt nameigk
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SpeciesH. sapiens (human), Synthetic
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Entrez GeneIGK (a.k.a. IGK@)
- Promoter CamKlla
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Tags
/ Fusion Proteins
- EGFP (C terminal on insert)
- Myc (C terminal on insert)
- PDGFR TM (C terminal on insert)
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer CaMKlla-F
- 3′ sequencing primer WPRE-R
- (Common Sequencing Primers)
Resource Information
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pAAV-CaMKIIα-igκ-mEGFP-TM was a gift from Sangkyu Lee (Addgene plasmid # 259745 ; http://n2t.net/addgene:259745 ; RRID:Addgene_259745) -
For your References section:
Remodeling synaptic connections via engineered neuron-astrocyte interactions. Kim SH, Won W, Kim GH, Kook YH, Son S, Choi S, Kang DY, Park MG, Choi YJ, Won SS, Shin J, Jeong Y, Lee KJ, Lee CJ, Lee S. Nat Commun. 2026 Apr 15;17(1):3490. doi: 10.1038/s41467-026-71440-w. 10.1038/s41467-026-71440-w PubMed 41986345