pCMV-igκ-mEGFP-TM
(Plasmid
#259743)
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PurposeA mammalian expression vector designed to express the mEGFP-tagged SynTrogo ligand in vitro under the control of the CMV promoter.
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 259743 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
Backbone
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Vector backbonepCMV
- Backbone size w/o insert (bp) 3983
- Total vector size (bp) 4979
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameigk-mEGFP-PDGFR TM
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SpeciesH. sapiens (human), Synthetic
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Entrez GeneIGK (a.k.a. IGK@)
- Promoter CMV
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Tags
/ Fusion Proteins
- EGFP (C terminal on insert)
- Myc (C terminal on insert)
- PDGFR TM (C terminal on insert)
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer CMV-F
- 3′ sequencing primer SV40-pAR
- (Common Sequencing Primers)
Resource Information
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCMV-igκ-mEGFP-TM was a gift from Sangkyu Lee (Addgene plasmid # 259743 ; http://n2t.net/addgene:259743 ; RRID:Addgene_259743) -
For your References section:
Remodeling synaptic connections via engineered neuron-astrocyte interactions. Kim SH, Won W, Kim GH, Kook YH, Son S, Choi S, Kang DY, Park MG, Choi YJ, Won SS, Shin J, Jeong Y, Lee KJ, Lee CJ, Lee S. Nat Commun. 2026 Apr 15;17(1):3490. doi: 10.1038/s41467-026-71440-w. 10.1038/s41467-026-71440-w PubMed 41986345