pMF1-JHS-105-mKate
(Plasmid #256134)

• Purpose: Sulfide biosensor plasmid expressing constitutive sqrR from Rhodobacter capsulatus and mKate2 from engineered sqrR-regulated promoter
• Depositing Lab: Benjamin Woolston
• Publication: Fernez et al ACS Synth Biol. 2025 Jun 20;14(6):2198-2207. doi: 10.1021/acssynbio.5c00124. Epub 2025 May 13.

Backbone

• Vector backbone: pSC101
• Total vector size (bp): 4711
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert 1

• Gene/Insert name: mKate2
• Species: Synthetic
• Insert Size (bp): 696
• Promoter: pSQR

Cloning Information for Gene/Insert 1

• Cloning method: Gene Synthesis
• 5′ sequencing primer: None

Gene/Insert 2

• Gene/Insert name: pSQR
• Insert Size (bp): 114
• Mutation: Includes rrnD1 UP element, and optimized RBS

Cloning Information for Gene/Insert 2

• Cloning method: Gene Synthesis
• 5′ sequencing primer: None

Gene/Insert 3

• Gene/Insert name: sqrR
• Species: Rhodobacter capsulatus
• Insert Size (bp): 375
• Mutation: Codon harmonized for expression in E. coli
• Promoter: J23105
• Tag / Fusion Protein:
  • 6xHis-SUMO (N terminal on insert)

Cloning Information for Gene/Insert 3

• Cloning method: Gene Synthesis
• 5′ sequencing primer: None

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Evrogen Limited Use Label License for FPs
• Industry Terms:
  • Not Available to Industry