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pIVT-EB3-mScarlet3
(Plasmid #256180)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 256180 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    pIVT
  • Vector type
    IVT mRNA expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    EB3-mScarlet3
  • Species
    H. sapiens (human)

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.64898/2026.01.02.697412 for bioRxiv preprint. Due to the difficulties of maintaining and sequencing long homopolymers, Addgene cannot guarantee the exact length of polyA sequence. The depositor noted that variations do not impact the function of these plasmids as scientists can re-add all polyA via primers when they PCR the backbone.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pIVT-EB3-mScarlet3 was a gift from Jennifer Lippincott-Schwartz (Addgene plasmid # 256180 ; http://n2t.net/addgene:256180 ; RRID:Addgene_256180)

  • For your References section:

    mRNAbow: A versatile gene expression system for multiplexed fluorescent imaging using optimized in vitro transcribed mRNA. Choi H, Halanych C, Kasberg W, Testa MD, Rubin-Elgressy S, Nguyen P, Walpita D, Tsang A, Cortes D, Song EY, Wu H, Weissman IL, Espinosa-Medina I, Satou C, Song JL, Matus DQ, Lippincott-Schwartz J. bioRxiv 2026.01.02.697412 10.64898/2026.01.02.697412
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