pPSntR
(Plasmid
#256231)
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Purpose(Empty Backbone) Plasmid for Plant STARR-seq with enhancer candidates in reverse orientation
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 256231 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
Backbone
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Vector backbonepPSup (Addgene # 149416)
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Backbone manufacturerFields Lab, Jores et al. 2020 doi: 10.1105/tpc.20.00155
- Backbone size (bp) 5426
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Modifications to backboneThe terminator of the GFP reporter gene in pPSup was replaced with a 35S terminator and the Golden Gate scars for the enhancer candidate insertion site were modified.
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Vector typePlant Expression
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Selectable markersBasta
Growth in Bacteria
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Bacterial Resistance(s)Spectinomycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Cloning Information
- Cloning method Golden Gate
Resource Information
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pPSntR was a gift from Stan Fields (Addgene plasmid # 256231)