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pDMJ2_SARS2_S_A.23_Q613
(Plasmid #256989)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 256989 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    pDMJ2
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    SARS-CoV-2 Spike
  • Species
    SARS-CoV-2
  • Mutation
    F157L, V367F
  • Entrez Gene
    S (a.k.a. GU280_gp02, spike glycoprotein)

Cloning Information

  • Cloning method Gene Synthesis

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2023.03.30.535005 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pDMJ2_SARS2_S_A.23_Q613 was a gift from Jeremy Luban (Addgene plasmid # 256989 ; http://n2t.net/addgene:256989 ; RRID:Addgene_256989)

  • For your References section:

    S:D614G and S:H655Y are gateway mutations that act epistatically to promote SARS-CoV-2 variant fitness. Yurkovetskiy L, Egri S, Kurhade C, Diaz-Salinas MA, Jaimes JA, Nyalile T, Xie X, Choudhary MC, Dauphin A, Li JZ, Munro JB, Shi PY, Shen K, Luban J. bioRxiv [Preprint]. 2023 Apr 24:2023.03.30.535005. doi: 10.1101/2023.03.30.535005. 10.1101/2023.03.30.535005 PubMed 37034621
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