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pAV 5S
(Plasmid #25708)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 25708 Standard format: Plasmid sent in bacteria as agar stab 1 $89

Backbone

  • Vector backbone
    pAV U6+27
  • Backbone manufacturer
    Engelke Lab
  • Backbone size w/o insert (bp) 6000
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    JM105
  • Growth instructions
    Provided in JM105
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    5S rRNA
  • Species
    H. sapiens (human)
  • Mutation
    Includes upstream sequences (-85) and the entire 5S rRNA gene

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site SalI (not destroyed)
  • 5′ sequencing primer hU6-F (5'-GAGGGCCTATTTCCCATGATT-3')
  • 3′ sequencing primer M13_puc_F
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The human 5S cassette was created by PCR amplifying the 85 nt upstream of the transcription start site through the entire 5S rRNA sequence using the ph5S 8544 plasmid (gift of Beth Moorfield) as template.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAV 5S was a gift from David Engelke (Addgene plasmid # 25708)

  • For your References section:

    Localized expression of small RNA inhibitors in human cells. Paul CP, Good PD, Li SX, Kleihauer A, Rossi JJ, Engelke DR. Mol Ther. 2003 Feb . 7(2):237-47. 10.1016/S1525-0016(02)00038-2 PubMed 12597912
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