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pMXs-3xFLAG-SLC25A39(aa72-106)-GFP-P2A-GFP-HA
(Plasmid #257559)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 257559 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    pMXs (Birsoy lab variant - see Comments)
  • Backbone manufacturer
    Cell Biolabs (original pMXs); modified in Birsoy lab
  • Vector type
    Mammalian Expression, Retroviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    SLC25A39
  • Species
    H. sapiens (human)
  • Mutation
    aa72-106
  • Entrez Gene
    SLC25A39 (a.k.a. CGI-69, CGI69)
  • Promoter 5' LTR (MMLV)
  • Tags / Fusion Proteins
    • 3xFLAG (N terminal on insert)
    • GFP-P2A-GFP-HA (C terminal on insert)

Cloning Information

  • Cloning method Unknown

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Mutation(s): aa72-106

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMXs-3xFLAG-SLC25A39(aa72-106)-GFP-P2A-GFP-HA was a gift from Kivanc Birsoy (Addgene plasmid # 257559 ; http://n2t.net/addgene:257559 ; RRID:Addgene_257559)

  • For your References section:

    Autoregulatory control of mitochondrial glutathione homeostasis. Liu Y, Liu S, Tomar A, Yen FS, Unlu G, Ropek N, Weber RA, Wang Y, Khan A, Gad M, Peng J, Terzi E, Alwaseem H, Pagano AE, Heissel S, Molina H, Allwein B, Kenny TC, Possemato RL, Zhao L, Hite RK, Vinogradova EV, Mansy SS, Birsoy K. Science. 2023 Nov 17;382(6672):820-828. doi: 10.1126/science.adf4154. Epub 2023 Nov 2. 10.1126/science.adf4154 PubMed 37917749
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