PurposeEntry vector with human U6 promoter driving mouse G alpha 12 miR30-based shRNA.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||25757||Standard format: Plasmid sent in bacteria as agar stab||1||$85|
This material is available to academics and nonprofits only.
Backbone manufacturerATCC 10326362
- Backbone size w/o insert (bp) 4310
Vector typeEntry vector
Growth in Bacteria
Bacterial Resistance(s)Gentamicin, 10 μg/mL
Gene/Insert nameG alpha 12 miR-shRNA
Alt nameG alpha 12
SpeciesM. musculus (mouse)
Insert Size (bp)22
Entrez GeneGna12 (a.k.a. Galpha12)
- Cloning method Restriction Enzyme
- 5′ cloning site attL1 (not destroyed)
- 3′ cloning site attL2 (not destroyed)
- 5′ sequencing primer LKO1-5
- 3′ sequencing primer n/a (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
Entry vector with U6-driven G alpha 12 miR-shRNA
G alpha 12 miR-shRNA: GCGACACCATCTTCGACAACAT
Addgene sequence shows that this plasmid is missing the EcoRI site.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pEN_hU6miR-G12-E was a gift from Iain Fraser (Addgene plasmid # 25757 ; http://n2t.net/addgene:25757 ; RRID:Addgene_25757)
For your References section:A single lentiviral vector platform for microRNA-based conditional RNA interference and coordinated transgene expression. Shin KJ, Wall EA, Zavzavadjian JR, Santat LA, Liu J, Hwang JI, Rebres R, Roach T, Seaman W, Simon MI, Fraser ID. Proc Natl Acad Sci U S A. 2006 Sep 12. 103(37):13759-64. 10.1073/pnas.0606179103 PubMed 16945906