PEA1-SLC25A39(C88C)
(Plasmid
#257594)
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PurposePrime editing for SLC25A39
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 257594 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
Backbone
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Vector backbonePEA1 (prime editing pegRNA expression vector)
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Vector typeMammalian Expression, CRISPR
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature30°C
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Growth Strain(s)NEB Stable
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameSLC25A39
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gRNA/shRNA sequencegtgcTCTGTATCTGTGCCCAAATGGTGC
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SpeciesH. sapiens (human)
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Insert Size (bp)20
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MutationC88C
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Entrez GeneSLC25A39 (a.k.a. CGI-69, CGI69)
- Promoter U6 (pegRNA)
Cloning Information
- Cloning method Unknown
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Mutation(s): C88C
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
PEA1-SLC25A39(C88C) was a gift from Kivanc Birsoy (Addgene plasmid # 257594 ; http://n2t.net/addgene:257594 ; RRID:Addgene_257594) -
For your References section:
Autoregulatory control of mitochondrial glutathione homeostasis. Liu Y, Liu S, Tomar A, Yen FS, Unlu G, Ropek N, Weber RA, Wang Y, Khan A, Gad M, Peng J, Terzi E, Alwaseem H, Pagano AE, Heissel S, Molina H, Allwein B, Kenny TC, Possemato RL, Zhao L, Hite RK, Vinogradova EV, Mansy SS, Birsoy K. Science. 2023 Nov 17;382(6672):820-828. doi: 10.1126/science.adf4154. Epub 2023 Nov 2. 10.1126/science.adf4154 PubMed 37917749