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pGL3-MYB-3'UTR
(Plasmid #25798)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 25798 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pGL3-control
  • Backbone manufacturer
    promega
  • Backbone size w/o insert (bp) 5256
  • Vector type
    Mammalian Expression, Luciferase

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    3'UTR of human MYB
  • Alt name
    c-myb
  • Alt name
    3'UTR MYB
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1191
  • GenBank ID
    NM_005375
  • Entrez Gene
    MYB (a.k.a. Cmyb, c-myb, c-myb_CDS, efg)
  • Tag / Fusion Protein
    • luciferase (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XbaI (not destroyed)
  • 3′ cloning site SalI (not destroyed)
  • 5′ sequencing primer n/a
  • 3′ sequencing primer n/a
  • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The c-myb 3'UTR sequence replaces the SV40 enhancer and SV40 poly(A) signal of pGL3-control

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGL3-MYB-3'UTR was a gift from Judy Lieberman (Addgene plasmid # 25798 ; http://n2t.net/addgene:25798 ; RRID:Addgene_25798)
  • For your References section:

    miR-34a contributes to megakaryocytic differentiation of K562 cells independently of p53. Navarro F, Gutman D, Meire E, Caceres M, Rigoutsos I, Bentwich Z, Lieberman J. Blood. 2009 Sep 3. 114(10):2181-92. 10.1182/blood-2009-02-205062 PubMed 19584398