Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||25803||Standard format: Plasmid sent in bacteria as agar stab||1||$85|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 5300
Vector typeMammalian Expression
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Copy numberHigh Copy
Gene/Insert namePQBP1 with Y65C mutation
SpeciesH. sapiens (human)
Insert Size (bp)1000
MutationTyrosine 65 within the WW domain of PQBP1 is changed to Cysteine
Entrez GenePQBP1 (a.k.a. MRX2, MRX55, MRXS3, MRXS8, NPW38, RENS1, SHS)
/ Fusion Proteins
- His (N terminal on insert)
- Xpress (N terminal on insert)
- Cloning method Restriction Enzyme
- 5′ cloning site BamH1 (unknown if destroyed)
- 3′ cloning site EcoR1 (unknown if destroyed)
- 5′ sequencing primer n/A
- 3′ sequencing primer n/A (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pcDNA4-His-Max-A-PQBP1-Y65C was a gift from Marius Sudol (Addgene plasmid # 25803 ; http://n2t.net/addgene:25803 ; RRID:Addgene_25803)
For your References section:Y65C missense mutation in the WW domain of the golabi-ito-hall syndrome protein PQBP1 affects its binding activity and deregulates pre-mRNA splicing. Tapia VE, Nicolaescu E, McDonald CB, Musi V, Oka T, Inayoshi Y, Satteson AC, Mazack V, Humbert J, Gaffney CJ, Beullens M, Schwartz CE, Landgraf C, Volkmer R, Pastore A, Farooq A, Bollen M, Sudol M. J Biol Chem. 2010 Apr 21. ():. 10.1074/jbc.M109.084525 PubMed 20410308