Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||25804||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 5500
Vector typeMammalian Expression
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Copy numberHigh Copy
SpeciesH. sapiens (human)
Insert Size (bp)1000
Entrez GenePQBP1 (a.k.a. MRX2, MRX55, MRXS3, MRXS8, NPW38, RENS1, SHS)
/ Fusion Protein
- 6xHA (N terminal on insert)
- Cloning method Restriction Enzyme
- 5′ sequencing primer none (Common Sequencing Primers)
A portion of this plasmid was derived from a plasmid made byDr. Mathieu Bollen, Co-author. Dr. Erica A. Golemis, Collaborator.
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
Several references within the article
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pcDNA3-HA-PQBP1 was a gift from Marius Sudol (Addgene plasmid # 25804 ; http://n2t.net/addgene:25804 ; RRID:Addgene_25804)
For your References section:Y65C missense mutation in the WW domain of the golabi-ito-hall syndrome protein PQBP1 affects its binding activity and deregulates pre-mRNA splicing. Tapia VE, Nicolaescu E, McDonald CB, Musi V, Oka T, Inayoshi Y, Satteson AC, Mazack V, Humbert J, Gaffney CJ, Beullens M, Schwartz CE, Landgraf C, Volkmer R, Pastore A, Farooq A, Bollen M, Sudol M. J Biol Chem. 2010 Apr 21. ():. 10.1074/jbc.M109.084525 PubMed 20410308