pRRL Jag1-mEmerald
(Plasmid
#258460)
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PurposeLentiviral expression of GFP-tagged JAG1
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 258460 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
Backbone
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Vector backbonepRRL
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Vector typeMammalian Expression, Lentiviral
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature30°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameJAG1
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SpeciesH. sapiens (human)
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Entrez GeneJAG1 (a.k.a. AGS, AGS1, AHD, AWS, CD339, CMT2HH, DCHE, HJ1, JAGL1)
- Promoter CMV
Cloning Information
- Cloning method Gibson Cloning
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pRRL Jag1-mEmerald was a gift from Matthew Kutys (Addgene plasmid # 258460 ; http://n2t.net/addgene:258460 ; RRID:Addgene_258460) -
For your References section:
The Notch1 intracellular domain orchestrates mechanotransduction of fluid shear stress. Singh T, Jacobs KA, Polacheck WJ, Kutys ML. Life Sci Alliance. 2026 Feb 5;9(4):e202503599. doi: 10.26508/lsa.202503599. Print 2026 Apr. 10.26508/lsa.202503599 PubMed 41644301