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Addgene

pCMV5 KSR1 S392A
(Plasmid #25976)

Full plasmid sequence is not available for this item.

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Item Catalog # Description Quantity Price (USD)
Plasmid 25976 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pCMV5
  • Backbone size w/o insert (bp) 4700
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Kinase suppressor of Ras 1
  • Alt name
    KSR1
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    2700
  • Mutation
    changed serine 392 to alanine
  • GenBank ID
    NM_013571
  • Entrez Gene
    Ksr1 (a.k.a. B-KSR1, D11Bhm183e, D11Bhm184e, Ksr, RSU2, hb, mKSR1)
  • Tag / Fusion Protein
    • Flag (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoR1 (not destroyed)
  • 3′ cloning site Kpn1 (not destroyed)
  • 5′ sequencing primer sequence
  • 3′ sequencing primer sequence
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    M. Therrien

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCMV5 KSR1 S392A was a gift from Rob Lewis (Addgene plasmid # 25976 ; http://n2t.net/addgene:25976 ; RRID:Addgene_25976)
  • For your References section:

    Phosphorylation regulates the nucleocytoplasmic distribution of kinase suppressor of Ras. Brennan JA, Volle DJ, Chaika OV, Lewis RE. J Biol Chem. 2002 Feb 15. 277(7):5369-77. 10.1074/jbc.M109875200 PubMed 11741955