Igk-vGFP(N)-TM-nMagHigh-tBFP
(Plasmid
#260315)
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PurposeGFP targeting extrabody N-terminal fragment
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Depositing Lab
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Depositing OrganizationKorea Advanced Institute of Science and Technology
Located in the Republic of Korea -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 260315 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
Backbone
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Vector backbonepDisplay
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameSplit GFP nanobody N-terminal fragment conjugated with nMagHigh domains on PDGFR transmembrane under KGC-ER2 signal sequence
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Alt namevhhGFP4
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SpeciesH. sapiens (human)
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Insert Size (bp)1710
- Promoter CMV
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Tag
/ Fusion Protein
- HA tags (N terminal on insert)
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer GAAATTTGTGATGCTATTGC
- (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Igk-vGFP(N)-TM-nMagHigh-tBFP was a gift from Won Do Heo (Addgene plasmid # 260315 ; http://n2t.net/addgene:260315 ; RRID:Addgene_260315) -
For your References section:
An extracellular, optogenetic antibody platform for stimulus-gated antigen recognition and modulation of cell behavior. Kwon E, Yu D, Yu J, Kim HJ, Jeong Y, Lee HK, Heo WD. Cell Chem Biol. 2026 Jun 18;33(6):848-861.e6. doi: 10.1016/j.chembiol.2026.04.006. Epub 2026 May 7. 10.1016/j.chembiol.2026.04.006 PubMed 42102802