Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||26032||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector typeEntry vector
Growth in Bacteria
Copy numberHigh Copy
SpeciesD. rerio (zebrafish)
Insert Size (bp)753
Entrez Geneeef1a1l1 (a.k.a. EFL1-, EFL1-alpha, chunp69, chunp6927, eef1a, ef1, ef1a, ik:tdsubc_2a3, ik:tdsubc_2b3, tdsubc_2, tdsubc_2a3, wu:fa91c0, wu:fa91c07, wu:fa94b0, wu:fa94b03, wu:fi13b0, wu:fi13b09, xx:tdsubc_2a3, xx:tdsubc_2b3)
- Cloning method Restriction Enzyme
- 5′ cloning site attL1 (not destroyed)
- 3′ cloning site attL2 (not destroyed)
- 5′ sequencing primer M13pUC-fwd
- 3′ sequencing primer M13rev (Common Sequencing Primers)
EF1alpha gene insert (also known as ef1a, accession number NM_131263) encompassing the TATA box, first non-coding exon 1, intron 1 and partial exon 2, 5′ of the ATG.
There are minor mismatches in the ef1a region. These changes should not affect plasmid function.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:512 pME-miR was a gift from Nathan Lawson (Addgene plasmid # 26032 ; http://n2t.net/addgene:26032 ; RRID:Addgene_26032)
For your References section:MicroRNA-mediated integration of haemodynamics and Vegf signalling during angiogenesis. Nicoli S, Standley C, Walker P, Hurlstone A, Fogarty KE, Lawson ND. Nature. 2010 Apr 22. 464(7292):1196-200. 10.1038/nature08889 PubMed 20364122