HDM-SARS2-Spike-delta21-Omicron-BA.5
(Plasmid
#260579)
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PurposeFor packaging SARS-CoV-2 Omicron BA.5 spike pseudotyped lentivirus
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Depositing Lab
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Depositing OrganizationChinese University of Hong Kong
Located in Hong Kong Special Administrative Region of China -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 260579 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
Backbone
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Vector backboneHDM
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameSARS2-Spike-delta21-Omicron BA.5
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SpeciesSARS-CoV-2
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MutationT191I/L24S/d25-27/d69-70/G142D/V213G/G339D/S371F/S373P/S375F/T376A/D405N/R408S/K417N/N440K/L452R/S477N/T478K/E484A/F486V/Q498R/N501Y/Y505H/D614G/H655Y/N679K/P681H/N764K/D796Y/Q954H/N969K
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Entrez GeneS (a.k.a. GU280_gp02, spike glycoprotein)
- Promoter CMV
Cloning Information
- Cloning method Ligation Independent Cloning
Resource Information
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
HDM-SARS2-Spike-delta21-Omicron-BA.5 was a gift from Bo Feng (Addgene plasmid # 260579 ; http://n2t.net/addgene:260579 ; RRID:Addgene_260579) -
For your References section:
Redirection of SARS-CoV-2 to phagocytes by intranasal sACE2-Fc as a universal decoy confers complete prophylactic protection. Wang J, Li J, Chin AWH, Luo B, Wei J, Qiu J, Ren J, Xia Y, Braun T, Poon LLM, Feng B. Elife. 2026 May 18;14:RP108883. doi: 10.7554/eLife.108883. 10.7554/eLife.108883 PubMed 42149106