I-SceI pB 5UAS::ZfSyGCaMP2
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||26125||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector backbonepBluescript II (KS)
- Backbone size w/o insert (bp) 3585
Vector typeZebrafish expression
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Copy numberHigh Copy
Gene/Insert nameSynaptophysin GCaMP2
SpeciesD. rerio (zebrafish)
Insert Size (bp)2262
Entrez Genesypb (a.k.a. zgc:136469, zgc:92837)
/ Fusion Protein
- GCaMP2 (C terminal on insert)
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRV (destroyed during cloning)
- 5′ sequencing primer M13-F (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
At both ends of the multiple cloning site of the original pBluescript II KS+, a I-SceI recognition site was cloned replacing the BssHII cutting sequence.
In the map, ORF frame 3 (1107-3368): zebrafish SyGCaMP2. Also, 5UAS is located 668-1034.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:I-SceI pB 5UAS::ZfSyGCaMP2 was a gift from Leon Lagnado (Addgene plasmid # 26125 ; http://n2t.net/addgene:26125 ; RRID:Addgene_26125)
For your References section:A genetically encoded reporter of synaptic activity in vivo. Dreosti E, Odermatt B, Dorostkar MM, Lagnado L. Nat Methods. 2009 Dec . 6(12):883-9. 10.1038/nmeth.1399 PubMed 19898484