pCAGGS-FGF10-EGFP
(Plasmid
#261286)
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PurposeExpresses EGFP-tagged mouse FGF10 in mammalian cells
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Depositing Lab
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Depositing OrganizationHarvard Medical School
Located in the United States of America -
Publication
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 261286 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
Backbone
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Vector backbonepCAGGS
- Total vector size (bp) 4900
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameFibroblast Growth Factor 10
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Alt nameFGF10
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SpeciesM. musculus (mouse)
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Insert Size (bp)1300
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Entrez GeneFgf10 (a.k.a. AEY17, Fgf-10, Fgf5a, Gsfaey17)
- Promoter CAG
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Tag
/ Fusion Protein
- EGFP (N terminal on insert)
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer pCAGGS-5
- (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byInsert synthesized by Twist Biosciences.
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://doi.org/10.64898/2026.06.16.732783 for bioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCAGGS-FGF10-EGFP was a gift from Cliff Tabin (Addgene plasmid # 261286 ; http://n2t.net/addgene:261286 ; RRID:Addgene_261286) -
For your References section:
Pitx2 modulates Fgf10 dosage to initiate asymmetric lung morphogenesis. Yan R, Helms J, Li P, Tabin CJ. bioRxiv 2026.06.16.732783 10.64898/2026.06.16.732783