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pMBP-SuCas12a2
(Plasmid #262217)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 262217 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    pMBP
  • Vector type
    Bacterial Expression, CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    SuCas12a2
  • Insert Size (bp)
    3699
  • Promoter T7
  • Tag / Fusion Protein
    • 10xHis-MBP (N terminal on insert)

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer GATGAAGCCCTGAAAGACGCGCAG
  • 3′ sequencing primer CTA GTT ATT GCT CAG CGG T
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMBP-SuCas12a2 was a gift from Juhong Chen (Addgene plasmid # 262217 ; http://n2t.net/addgene:262217 ; RRID:Addgene_262217)

  • For your References section:

    Indiscriminate Trans-Cleavage Activity of CRISPR/SuCas12a2 Enables Sensitive Detection of SARS-CoV-2. Liu Z, He Y, Lin X, Pal T, Pfeilsticker A, Liu BM, Chen J. ACS Sens. 2026 Jul 24;11(7):5397-5403. doi: 10.1021/acssensors.6c00990. 10.1021/acssensors.6c00990 PubMed 42399104
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