|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||26260||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 9110
Vector typeBacterial Expression, Insect Expression ; Drosophila transgenesis
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Copy numberHigh Copy
SpeciesS. cerevisiae (budding yeast)
Insert Size (bp)2965
Entrez GeneGAL4 (a.k.a. YPL248C, GAL81)
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site SacI (not destroyed)
- 5′ sequencing primer CAGTGCACGTTTGCTTGTTGA (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
Brand and Perrimon. Development. 1993 Jun;118(2):401-15.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pXL-BACII-attPGAL4LWL was a gift from Tom Clandinin (Addgene plasmid # 26260 ; http://n2t.net/addgene:26260 ; RRID:Addgene_26260)
For your References section:A versatile in vivo system for directed dissection of gene expression patterns. Gohl D, Silies M, Gao X, Bhalerao S, Luongo F, Lin CC, Potter C, Clandinin T. Nature Methods (2011) doi:10.1038/nmeth.1561 10.1038/nmeth.1561