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pQCXIG (w497-1)
(Plasmid #26286)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 26286 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pQCXIP
  • Backbone manufacturer
    Clontech
  • Backbone size (bp) 7116
  • Vector type
    Mammalian Expression, Retroviral
  • Selectable markers
    GFP

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    TOP10F', 37oC.
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    no insert
  • Alt name
    Enhanced Green Fluorescent Protein

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Not I (not destroyed)
  • 3′ cloning site Eco RI (not destroyed)
  • 5′ sequencing primer CMVforw
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

A cDNA or miRNA can be cloned downstream of the CMV promoter. There is an IRES sequence followed by the GFP cDNA so transduced cells will be fluorescent.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pQCXIG (w497-1) was a gift from Eric Campeau (Addgene plasmid # 26286 ; http://n2t.net/addgene:26286 ; RRID:Addgene_26286)