|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||26286||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size (bp) 7116
Vector typeMammalian Expression, Retroviral
Growth in Bacteria
Growth instructionsTOP10F', 37oC.
Copy numberHigh Copy
Gene/Insert nameno insert
Alt nameEnhanced Green Fluorescent Protein
- Cloning method Restriction Enzyme
- 5′ cloning site Not I (not destroyed)
- 3′ cloning site Eco RI (not destroyed)
- 5′ sequencing primer CMVforw (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
A cDNA or miRNA can be cloned downstream of the CMV promoter. There is an IRES sequence followed by the GFP cDNA so transduced cells will be fluorescent.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pQCXIG (w497-1) was a gift from Eric Campeau (Addgene plasmid # 26286 ; http://n2t.net/addgene:26286 ; RRID:Addgene_26286)