|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||26287||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size (bp) 7072
Vector typeMammalian Expression, Retroviral
Growth in Bacteria
Growth instructionsTOP10F', 37oC
Copy numberHigh Copy
Gene/Insert nameno insert
Alt nameRed Fluorescent Protein 2
- Cloning method Restriction Enzyme
- 5′ cloning site Age I (not destroyed)
- 3′ cloning site Bam HI (not destroyed)
- 5′ sequencing primer CMVforw (Common Sequencing Primers)
A portion of this plasmid was derived from a plasmid made byDsRed2 cDNA was derived from pIRES DsRed 2 plasmid (Clontech)
Terms and Licenses
- Not Available to Industry
A cDNA or miRNA can be inserted downstream of the CMV promoter. There is an IRES with the red fluorescent protein downstream so the transduced cells will be red.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pQCXIR (w498-1) was a gift from Eric Campeau (Addgene plasmid # 26287 ; http://n2t.net/addgene:26287 ; RRID:Addgene_26287)