|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||26298||Standard format: Plasmid sent in bacteria as agar stab||1||$85|
This material is available to academics and nonprofits only.
Vector backbonepBluescript II KS+
- Backbone size (bp) 4569
Vector typeTrypanosome in-situ tagging vector
Selectable markersNeomycin (select with G418)
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
/ Fusion Protein
- triple cMyc and triple HA in tandem (C terminal on insert)
- Cloning method Restriction Enzyme
- 5′ sequencing primer n/a (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
Modifications giving different epitope tags and/or selectable markers made by members of the Cross lab to plasmids based on Oberholzer,M., Morand,S., Kunz,S., and Seebeck,T.
A vector series for rapid PCR-mediated C-terminal in-situ tagging of T. brucei genes. Mol. Biochem. Parasitol. 145, 2005, 117-120.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pMOTag4HM was a gift from George Cross (Addgene plasmid # 26298 ; http://n2t.net/addgene:26298 ; RRID:Addgene_26298)