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pRL-TK 3'FBXW7 UTR
(Plasmid #26649)

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Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 26649 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pRL-TK
  • Backbone manufacturer
    Promega
  • Backbone size w/o insert (bp) 4045
  • Vector type
    Mammalian Expression, Luciferase

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    FBXW7
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1571
  • Mutation
    Contains only the 3'UTR of FBXW7, nucleotides 1-1571 following stop codon.
  • Entrez Gene
    FBXW7 (a.k.a. AGO, CDC4, FBW6, FBW7, FBX30, FBXO30, FBXW6, SEL-10, SEL10, hAgo, hCdc4)
  • Tag / Fusion Protein
    • Luciferase (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XbaI (not destroyed)
  • 3′ cloning site ApaI (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer EBV-Rev
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry

Depositor Comments

Please note that Addgene's sequencing results identified nucleotide mismatches when compared to GenBank reference sequence NM_018315.4 at basepair #'s 2389, 2663, 2666, 2829 and 3526.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pRL-TK 3'FBXW7 UTR was a gift from Alex Minella (Addgene plasmid # 26649 ; http://n2t.net/addgene:26649 ; RRID:Addgene_26649)
  • For your References section:

    MicroRNA-223 regulates cyclin E activity by modulating expression of F-box and WD-40 domain protein 7. Xu Y, Sengupta T, Kukreja L, Minella AC. J Biol Chem. 2010 Sep 7. ():. 10.1074/jbc.M110.152306 PubMed 20826802