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pcDNAz-mBACEflag (C474A/C478A/C482A/C485A)
(Plasmid #26736)

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Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 26736 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pcDNA3.1zeo (-)
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5014
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    BACE
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    1504
  • Mutation
    Cys 474, 478, 482, and 485 mutated to Ala
  • Entrez Gene
    Bace1 (a.k.a. ASP2, Bace)
  • Tag / Fusion Protein
    • FLAG (C terminal on insert)

Cloning Information

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Wt Bace1 received from Nabil Seidah, IRCM, Montreal
  • Article Citing this Plasmid

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Note that the F318L mutation present in the original Wt Bace1 from the Seidah lab is also present in this plasmid. The mutation is not known to affect function.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNAz-mBACEflag (C474A/C478A/C482A/C485A) was a gift from Gopal Thinakaran (Addgene plasmid # 26736 ; http://n2t.net/addgene:26736 ; RRID:Addgene_26736)
  • For your References section:

    Alzheimer disease Abeta production in the absence of S-palmitoylation-dependent targeting of BACE1 to lipid rafts. Vetrivel KS, Meckler X, Chen Y, Nguyen PD, Seidah NG, Vassar R, Wong PC, Fukata M, Kounnas MZ, Thinakaran G. J Biol Chem. 2009 Feb 6. 284(6):3793-803. 10.1074/jbc.M808920200 PubMed 19074428