Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||26869||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector typeMammalian Expression, Luciferase
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert nameHes5 promoter
SpeciesM. musculus (mouse)
Insert Size (bp)760
Entrez GeneHes5 (a.k.a. bHLHb3, bHLHb38)
/ Fusion Protein
- Luciferase (C terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ sequencing primer n/a
- 3′ sequencing primer Luc_N_Rev (Common Sequencing Primers)
Contains the Hes5 promoter (-685 to +28).
C->T mutation in the center of the promoter does affect function.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pHes5p-luc was a gift from Nicholas Gaiano (Addgene plasmid # 26869 ; http://n2t.net/addgene:26869 ; RRID:Addgene_26869)
For your References section:Differential Notch signalling distinguishes neural stem cells from intermediate progenitors. Mizutani K, Yoon K, Dang L, Tokunaga A, Gaiano N. Nature. 2007 Sep 20. 449(7160):351-5. 10.1038/nature06090 PubMed 17721509