CBFRE (mt) EGFP
Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||26870||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
Backbone manufacturerGaiano Lab
Vector typeMammalian Expression, Adenoviral
Growth in Bacteria
Copy numberHigh Copy
Alt nameCBF1-responsive element
MutationThe mutated CBF1 binding site: GATCTGGTGTAAA CACGGGCTTGGAAAAAATTTATG
/ Fusion Protein
- EGFP (C terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ sequencing primer SV40pro-F
- 3′ sequencing primer EGFP-N (Common Sequencing Primers)
Terms and Licenses
Article Citing this Plasmid
CBFRE: four (mutated) CBF1-binding elements from EBV (see Hsieh MCB 1996, 16:952) and the basal simian virus 40 (SV40) promoter upstream of EGFP. The sequence of the mutated CBF1 binding element is (GATCTGGTGTAAA
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:CBFRE (mt) EGFP was a gift from Nicholas Gaiano (Addgene plasmid # 26870 ; http://n2t.net/addgene:26870 ; RRID:Addgene_26870)
For your References section:Differential Notch signalling distinguishes neural stem cells from intermediate progenitors. Mizutani K, Yoon K, Dang L, Tokunaga A, Gaiano N. Nature. 2007 Sep 20. 449(7160):351-5. 10.1038/nature06090 PubMed 17721509