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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 27234 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepEF-BOS
- Backbone size w/o insert (bp) 5500
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameLGP2
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Alt nameDHX58
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Alt nameD11LGP2
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Alt nameRIG-I-like receptor LGP2
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SpeciesH. sapiens (human)
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Insert Size (bp)2040
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GenBank IDNP_077024
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Entrez GeneDHX58 (a.k.a. D11LGP2, D11lgp2e, LGP2, RLR-3)
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Tags
/ Fusion Proteins
- Flag (C terminal on backbone)
- His6 (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Xho1 (unknown if destroyed)
- 3′ cloning site BglII/BamH1 (unknown if destroyed)
- 5′ sequencing primer EF1a-F (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pEF-BOS LGP2 was a gift from Kate Fitzgerald (Addgene plasmid # 27234) -
For your References section:
The RNA helicase Lgp2 inhibits TLR-independent sensing of viral replication by retinoic acid-inducible gene-I. Rothenfusser S, Goutagny N, DiPerna G, Gong M, Monks BG, Schoenemeyer A, Yamamoto M, Akira S, Fitzgerald KA. J Immunol. 2005 Oct 15. 175(8):5260-8. PubMed 16210631